Subcellular Fractionation: Primary Chronic Lymphocytic Leukemia Cells-Preview
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Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking - a 2 minute Preview of the Experimental Protocol
Jodie Hay, Michael W. Moles, Jennifer Cassels, Alison M. Michie
University of Glasgow, Paul O'Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences;
This protocol enables the optimization and subsequent efficient generation of nuclear and cytoplasmic fractions from primary chronic lymphocytic leukemia cells. These samples are used to determine protein localization as well as changes in protein trafficking that take place between the nuclear and cytoplasmic compartments upon cell stimulation and drug treatment.
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