Subcellular Fractionation: Primary Chronic Lymphocytic Leukemia Cells-Preview

Watch the Full Video at https://www.jove.com/v/60426/subcellular-fractionation-primary-chronic-lymphocytic-leukemia-cells?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022.

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking - a 2 minute Preview of the Experimental Protocol

Jodie Hay, Michael W. Moles, Jennifer Cassels, Alison M. Michie
University of Glasgow, Paul O'Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences;

This protocol enables the optimization and subsequent efficient generation of nuclear and cytoplasmic fractions from primary chronic lymphocytic leukemia cells. These samples are used to determine protein localization as well as changes in protein trafficking that take place between the nuclear and cytoplasmic compartments upon cell stimulation and drug treatment.

Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel: https://www.youtube.com/c/JoVE....JournalofVisualizedE