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Merkel Cell Polyomavirus Infection and Detection | Protocol Preview
Watch the Full Video at https://www.jove.com/v/58950/merkel-cell-polyomavirus-infection-and-detection?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022.
Merkel Cell Polyomavirus Infection and Detection - a 2 minute Preview of the Experimental Protocol
Wei Liu, Nathan A. Krump, Christopher B. Buck, Jianxin You
University of Pennsylvania, Department of Microbiology, Perelman School of Medicine; National Cancer Institute, Laboratory of Cellular Oncology;
Here, we present a protocol to infect primary human dermal fibroblast with MCPyV. The protocol includes isolation of dermal fibroblasts, preparation of MCPyV virions, virus infection, immunofluorescence staining, and fluorescence in situ hybridization. This protocol can be extended for characterizing MCPyV-host interactions and discovering other cell types infectable by MCPyV.
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