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Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format - a 2 minute Preview of the Experimental Protocol

Tomoaki Kahyo, Haruhiko Sugimura
Hamamatsu University School of Medicine, Department of Tumor Pathology;

Digital polymerase chain reaction (PCR) is a useful tool for the high-sensitivity detection of single nucleotide variants and DNA copy number variants. Here, we demonstrate key considerations for measuring rare variants in the human genome using digital PCR with the chip-in-a-tube format.

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Most cases of colorectal cancer are linked to mutational inactivation of the Adenomatous polyposis coli (APC) tumour suppressor. Here Buchi Okereafor talks to author Mariann Bienz about her Open Biology paper "The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled" and the role APC plays in the development of colorectal cancer.

See http://rsob.royalsocietypublis....hing.org/content/1/3 for the full paper

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https://www.patreon.com/SeeHearSayLearn , http://www.youtube.com/c/SeeHe....arSayLearn?sub_confi In this video series we'll run through a large number of Genetic Disorders. The first step in studying anything is first understanding the correct pronunciation, and first impressions are everything. Say it right first and you'll save yourself mental anguish in trying to correct a pronunciation remembered inaccurately.

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The famous anatomy coloring book: http://amzn.to/2twlkQB
The famous Netter Anatomy book: http://amzn.to/2uq2dvL
- and the Netter Coloring book: http://amzn.to/2t6FsJz

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SeeHearSayLearn.com presents a series of videos to get you speaking and learning languages such as English, Spanish / Espanol, French, German, Albanian, Arabic, and more. We are working hard to get our videos uploaded. We provide you with word pronunciations, definitions, translations, stories, rhymes, riddles, jokes, tongue twisters, and anything that will help bridge the gap between your current fluency to your desired proficiency and understanding. Whether you're just learning or trying to bolster your intellectual quotient into a new stratosphere of concise and succinct communications, allocating the proper verbiage could be paramount to illustrating a picture for the recipient or merely shoving drab nondescript sounds of failure down their auditory meatuses. Run on sentence you say? I'd agree. Utilizing big complicated words isn't usually the most effective form of communication, but adapting your language to your recipient will be the most effective way to transfer your thoughts. Having a wide array of tools for each project will allow you to tailor your message for the most effect and efficient use of your time. To write, read, and listen to language takes fewer words than you might imagine. In each language, you could likely get away with understanding a few thousand words and be completely comfortable with many different language settings. Why even a few hundred can get you quite far.

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This is the start of my v-blog where I'll discuss what it's like to live with a rare genetic disease. I'll cover various topics from what it is, how a person gets it and how its "treated".

I want to give a shout-out to http://www.youtube.com/user/FAPulousTV, I probably would have never thought to speak out if I hadn't magically come across her videos.

Symptoms, risk factors and treatments of Familial adenomatous polyposis (Medical Condition)

Familial adenomatous polyposis is an inherited condition in which numerous adenomatous polyps form mainly in the epithelium of the large intestine

This video contains general medical information If in doubt, always seek professional medical advice.
The medical information is not advice and should not be treated as such. The medical information is provided without any representations or warranties, express or implied. We do not warrant or represent that the medical information on this websiteis true, accurate, complete, current or non-misleading

Music: 'Undaunted' Kevin Macleod CC-BY-3.0
Source/Images: "Familial adenomatous polyposis" CC-BY-2.5 https://www.freebase.com/m/046k0y