Retroperitoneal Lymphadenopathy From VX2 Cells : Endometrial Cancer | Protocol Preview

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An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells - a 2 minute Preview of the Experimental Protocol

Lauren Philp, Harley Chan, Marjan Rouzbahman, Ariana Rostami, Lili Ding, Scott V. Bratman, Margarete K. Akens, Jonathan C. Irish, Marcus Q. Bernardini, Gang Zheng
University of Toronto, Institute of Medical Science; University of Toronto, Department of Obstetrics and Gynecology; University Health Network, Guided Therapeutics Laboratory, TECHNA Institute; University Health Network, Department of Pathology, Princess Margaret Cancer Center; University of Toronto, Department of Medical Biophysics; University Health Network, Princess Margaret Cancer Center; University Health Network, Techna Institute; University of Toronto, Department of Surgery; University of Toronto, Department of Otolaryngology, Head and Neck Surgery; University Health Network, Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center; University Health Network, Princess Margaret Cancer Center;

This protocol presents a standardized method to grow VX2 cells in culture and to create an orthotopic VX2 model of endometrial cancer with retroperitoneal lymph node metastases in rabbits. Orthotopic endometrial cancer models are important for the pre-clinical study of novel imaging modalities for the diagnosis of lymph node metastases.

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• Category

  Endometrial Cancer