Osteosarcoma Modeling using Stem Cells | Protocol Preview
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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells - a 2 minute Preview of the Experimental Protocol
Ruoji Zhou, An Xu, Jian Tu, Mo Liu, Julian A Gingold, Ruiying Zhao, Dung-Fang Lee
The University of Texas Health Science Center at Houston, Department of Integrative Biology and Pharmacology, McGovern Medical School; The University of Texas MD Anderson Cancer Center UTHealth, Graduate School of Biomedical Sciences; The First Affiliated Hospital of Sun Yat-sen University, Department of Musculoskeletal Oncology; Cleveland Clinic Foundation, Women's Health Institute; The University of Texas Health Science Center at Houston, Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases; The University of Texas Health Science Center at Houston, Center for Precision Health, School of Biomedical Informatics and School of Public Health;
Here, we present a protocol for the generation of induced pluripotent Stem Cells (iPSCs) from Li-Fraumeni Syndrome (LFS) patient derived fibroblasts, differentiation of iPSCs via mesenchymal stem cells (MSCs) to osteoblasts, and modeling in vivo tumorigenesis using LFS patient-derived osteoblasts.
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